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Effect of body fat distribution on the physiological response to a dietary fat intervention
Author(s) -
Graaf Albert Arjaan,
Pasman Wilrike,
Schalkwijk Daan,
Freidig Andreas,
Hendriks Henk,
Vaes Wouter,
Verheij Elwin,
Bobeldijk Ivana
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.350.6
Subject(s) - adipose tissue , polyunsaturated fatty acid , medicine , endocrinology , fatty acid , chemistry , meal , biochemistry
We conducted a study to examine the effect of body fat distribution on the physiological response to a dietary fat intervention with medium chain fatty acids (MCFA) vs long‐chain fatty acids (PUFA) in humans. The physiological response was evaluated as fatty acid kinetics (plasma and subcutaneous fat appearance), plasma lipoprotein profiles and clinical chemistry parameters. In a cross‐over trial, 12 male subjects (age 51±7 y; BMI 28.5±0.8 kg.m‐2), divided in 2 groups according to waist‐hip ratio, were supplemented 60 grams/day MCFA (C8:0, C12:0) or PUFA (C18:2n‐6) for three weeks, with a wash‐out period between supplementation periods of six weeks. Fatty acid metabolism kinetics were studied by supplying [1,2,3,4‐ 13 C 4 ]C8:0 and [U‐ 13 C 18 ]C18:2n‐6 tracers together with a meal on the test day at the end of each intervention period. Samples taken at different time points after tracer administration were analyzed by GC‐C‐IRMS for concentration‐ and isotopic analysis. A clear treatment effect on fat metabolism was concluded from lipoprotein profiles as well as subcutaneous fat composition data from adipose tissue biopts. From the stable isotope data we conclude the presence of differences in acute fatty acid uptake influenced by treatment and fat disposition, that are moreover different in upper body obese vs lower body obese.