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Involvement of Sep15 family members in immune response
Author(s) -
Kasaikina Marina V,
Fomenko Dmitry E,
Jones Clinton J,
Reddy Jay,
Hatfield Dolph L.,
Gladyshev Vadim N
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.346.8
Subject(s) - biology , selenoprotein , immune system , cd8 , mhc class i , major histocompatibility complex , immunology , biochemistry , glutathione peroxidase , superoxide dismutase , antioxidant
Selenium and selenoproteins are known to have immunomodulatory properties. 15 kDa selenoprotein (Sep15) was previously shown to interact with UDP‐glucose:glycoprotein glucosyltransferase, a protein involved in the calnexin quality control cycle for many secreted glycoproteins, including proteins with the immune protection function (e.g., MHC class I receptor). In this study, we examined the resistance of Sep15 knockout mice (Sep15 KO) to viral infection and autoimmune disease. In both cases the development of the disease in the Sep15 KO group was different from that in wild type mice, and resulted in higher severity of symptoms and higher percentage of death cases. Examination of immunological parameters in mouse spleenocytes, including MHC class I and II, and CD4/CD8 population of T cells, did not show significant differences between WT and Sep15 KO groups. However, cell culture experiments with Sep15 KO MEFs demonstrated a decrease in â2‐microglobulin subunit when Sep15 KO was combined with deficiency in a distant Sep15 homolog, Selenoprotein M. Altogether, these data show the role of Sep15 family members in the immune function.