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Genetic Determinants of Responses to Selenium Supplementation
Author(s) -
Combs Gerald F.,
Zeng Huawei,
Jackson Matthew I.,
Johnson Luann K.,
Hoeg Antonia,
Schomburg Lutz,
Davis Cindy D.,
Milner John A.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.346.3
Subject(s) - selenoprotein p , gpx1 , selenoprotein , gpx3 , phospholipid hydroperoxide glutathione peroxidase , chemistry , glutathione peroxidase , genotype , glutathione , buccal swab , gpx4 , peroxidase , medicine , selenium , microbiology and biotechnology , endocrinology , biochemistry , biology , enzyme , gene , organic chemistry
In a cohort of healthy adults (106 M, 155 W) in eastern North Dakota, we determined the relationships of five biomarkers of selenium (Se) status (plasma Se, serum selenoprotein P [SePP], plasma glutathione peroxidase [GPX3] activity, buccal cell Se, urine Se) to genotype for four selenoproteins (cytosolic glutathione peroxidase [GPX1], phospholipid hydroperoxide glutathione peroxidase [GPX4], the 15 kD selenoprotein [SeP15], SePP) and two glutathione S‐transferases (GST‐M1, GST‐T1). The cohort had plasma Se of 141.5±23.7 (SD) ng/ml, SePP lof 3.55 (CI 2.61, 4.51) mg/L, GPX3 activities of 3.64±0.54 nmoles NADPH/min/mg protein, buccal cell Se of 10.2±6.5 ng Se/mg protein, and urinary Se of 58.2±21.5 ng Se/mg creatinine. Plasma Se was significantly related to GPX3 genotype (198Leu/Leu: 135.7 a± 19.0 ng/ml; 198Leu/Pro: 139.2 a,b± 23.8 ng/ml; 198 Pro/Pro: 145.9 b± 24.4 ng/ml; P<0.05). Buccal cell Se was significantly related to SeP15 genotype (C/C: 8.77 a [5.85, 13.14]; T/C: 7.65 b [5.06, 11.57]; T/T: 8.27 a,b [6.16, 11.10] P<0.05). SePP was significantly related to SePP genotype at a polymorphism in the 3′‐UTR region (A/A: 3.49 a,b [2.91, 4.18]; G/A: 3.24 a [2.44, 4.31]; G/G: 3.62 b [2.75, 4.76]), but not one in the coding region (A234T). No other effects were detected. These results show that genetic polymorphisms can significantly affect some biomarkers used to assess Se status.