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Identification of bacterial species with phenolic‐degrading activity in the human oral cavity
Author(s) -
Ye Zhong,
Rieser Erinn,
Li Li,
Hendrich Suzanne
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.345.6
Subject(s) - caffeic acid , naringenin , rutin , myricetin , saliva , chemistry , quercetin , food science , luteolin , biochemistry , flavonoid , antioxidant , kaempferol
We hypothesized that human oral bacteria had significant but highly variable phenolic degradation capability, which may influence ability of these compounds to prevent periodontal disease. Caucasian (2 men, 7 women) and Asian (3 men, 8 women)subjects who had no dental disease and were not taking supplements provided 5‐7mL saliva before toothbrushing. Rutin, caffeic acid, daidzein, quercetin, naringenin, luteolin and myricetin (each 100μM)were incubated with saliva in anaerobic BHI media at 37°C. Phenolics were measured at 0, 3, 6, 9, 12 and 24h by HPLC. Saliva bacterial 16S rRNA gene sequences were amplified and separated. Saliva degradation rates were caffeic acid/rutin > quercetin/ myricetin/naringenin > luteolin >daidzein (p < 0.05). High & low degradation rate clusters were found for rutin, caffeic acid and naringenin; degradation rates of these compounds were significantly correlated with each other. DNA bands from high degraders of caffeic acid were concordant with Streptomyces coelicolor and S. avermitilis , and Lactobacillus brevis/L. reuteri for high degraders of rutin. These species may affect human oral degradation of these phenolics and prevention of gum disease. (Funded by Nutrition & Wellness Res. Ctr, Iowa State University).