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Hepatic gene profiling prior to puberty reveals greater female predominant expression of lipogenic genes
Author(s) -
Shankar Kartik,
Ronis Martin J,
Badger Thomas M
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.343.6
Subject(s) - fatty acid synthase , medicine , endocrinology , biology , fatty acid synthesis , gene expression , fold change , fatty acid , lipid metabolism , gene , biochemistry
We evaluated changes in transcriptomic profiles in livers of pre‐peubertal rats using Affymetrix microarrays. Female Sprague‐Dawley rats were fed a phytoestrogen free, AIN‐93G‐based diet containing casein as the sole protein source from gestation day 4 and offspring were weaned onto the same diet. Male and female offspring were sacrificed on PND25 and hepatic gene expression assessed using the Rat 230 2.0 GeneChip microarrays. Data were analyzed using GeneSpring Gx7.3 and Ingenuity Pathway Analyses and transcripts that changed ±1.8‐fold (p <0.05) were enriched. Expression of 60 transcripts was significantly altered between genders. Among these, genes involved in lipid biosynthesis and elongation, adiponutrin (11‐fold), fatty acid synthase (4‐fold), ATP citrate lyase (2.9‐fold), ELOVL6 (5‐fold), acetyl‐coA carboxylase (2‐fold) and thyroid hormone responsive Spot 14 (2‐fold) were increased in females compared to males. Importantly, mRNA expression of the master lipogenic factor SREBP‐1 was increased 2.2‐fold in female liver. In addition, genes regulating fatty acid oxidation, Cpt1a, CYP4A14 and Slc22a5 and cholesterol transporters Abcg5, Abcg8 were lower in female livers (p<0.05). These results indicate significant gender differences in SREBP‐1 regulated lipogenic gene expression prior to divergence in major sex hormones at puberty. Support ARS‐CRIS 6251‐51000‐005‐00D.