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Old obese GPX1 overexpressing mice do not develop overt type 2 diabetes
Author(s) -
Roneker C A,
Yan X,
Huang L,
Vatamaniuk M,
Lei X G
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.338.4
Subject(s) - medicine , hyperinsulinemia , endocrinology , insulin resistance , type 2 diabetes , insulin , obesity , diabetes mellitus
Previously we reported that the GPX1 overexpressing mice (OE) developed a metabolic syndrome‐like phenotype at 6‐months of age. Subsequent characterization illustrated hyperinsulinemia as the primary effect of GPX1 overproduction. The objective of this study was to determine if the long‐term obesity and insulin resistance led to overt type 2 diabetes at old age. Five OE and wild‐type (WT) mice each were fed a Se‐adequate diet until 1 year of age and used for glucose and insulin analysis. Compared with the WT, the OE mice were 44% heavier (36.0 ± 4.2 vs. 51.9 ± 3.8 g, P < 0.05), had 5‐fold greater fasting plasma insulin concentration (0.21 ± 0.13 vs. 1.45 ± 0.33 ng/ml, P< 0.05), showed 50% less plasma glucose decrease (P < 0.05) at 30, 60, and 240 min after an injection of insulin (1 U/kg), and secreted 4 to 8 times more (P < 0.05) insulin at 15, 30, and 60 min after an injection of glucose (2 g/kg). However, the old OE mice exhibited only mild fasting hyperglycemia (100 ± 15 vs.144 ± 20 mg/dl) and improved glucose clearance, without glucosuria. In conclusion, the old OE mice seemed to be a novel obesity model that might be resistant to the development of overt type 2 diabetes (NIH DK53018).