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Protective effects of polyphenolic compounds on oxidative stress‐induced cytotoxicity in PC12 cells
Author(s) -
Crispo James A.G.,
Ansell D.,
Piché M.,
Eibl J.,
Khaper N.,
Ross G. M.,
Tai T.C.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.337.1
Subject(s) - viability assay , oxidative stress , gallic acid , reactive oxygen species , polyphenol , myricetin , chemistry , antioxidant , pharmacology , methyl gallate , biochemistry , mtt assay , quercetin , cell growth , cell , biology , kaempferol
Elevated reactive oxygen species (ROS) have been implicated in the pathogenesis of many diseases, including atherosclerosis and Parkinson's. Foods rich in polyphenols such as fruits, wines and teas have proven to decrease oxidative stress. Further, antioxidant properties of polyphenols increase cell viability under oxidative stress conditions and increase overall health. In the current study 12 polyphenols were screened for their ability to increase viability in PC12 cells subject to oxidative stress induced via cobalt chloride (CoCl 2 ) and hydrogen peroxide (H 2 O 2 ). Results from MTT assays show that pre‐treatment with 50µM methyl gallate increases cell viability by 26.6% (P<0.01) under H 2 O 2 stress and does not increase viability under CoCl 2 stress. Pre‐treatment with 100µM epigallocatechin gallate (EGCG) shows a 63.4% (P<0.01) increase in viability compared to control, however shows no viability increase in stress conditions. Treatments with 50µM myricetin and gallic acid show no effect on basal viability and decrease viability when delivered as a pre‐treatment prior to H 2 O 2 exposure. Remaining polyphenols did not alter basal viability or increase viability under stress. Lastly, DCFDA determination of intracellular ROS confirms the ability of screened polyphenols to significantly reduce ROS levels. These results suggest that methyl gallate and EGCG may have potential therapeutic properties.