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Choline intake, plasma riboflavin and the PEMT G5465A genotype predict plasma homocysteine in folate deficient Mexican American men with the MTHFR 677TT genotype
Author(s) -
Caudill Marie A,
Dellschaft Neele,
Hinkis Sabrina,
Ivanov Alexandre A,
NashBarboza Susan,
Randall Katharine E,
Jackson Brandi,
Solomita Gi,
Vermeylen Francoise
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.335.1
Subject(s) - methylenetetrahydrofolate reductase , genotype , homocysteine , medicine , creatinine , choline , endocrinology , biology , genetics , gene
We previously showed that provision of the folate RDA and either 300, 550, 1100, or 2200mg choline/day resulted in diminished folate status and a tripling of plasma total homocysteine (tHcy) in men with the MTHFR 677TT genotype However, the substantial variation in tHcy within the 677TT genotype implied that several factors were interacting with this genotype to affect homocysteine. This study sought to identify the main predictors of wk‐12 plasma tHcy, alone and together with the MTHFR C677T genotype (29 TT, 31 CC), using linear regression analysis. A basic model explaining 82.5% of the variation (i.e., adjusted R2 =0.825) was constructed. Within the 677TT genotype, serum folate (P=0.005) and plasma riboflavin (P=0.002) were strong negative predictors (inversely related) explaining 12% and 15% of the variation in tHcy; whereas choline intake (P=0.003) and serum creatinine (P<0.001) were strong positive predictors explaining 19% and 25% of the variation. None of these variables, except creatinine (P=0.021), correlated with tHcy within the 677CC genotype. When creatinine was not in the model, the PEMT 5465G>A variant predicted lower tHcy (P<0.001); an effect confined to the MTHFR 677TT genotype. Thus, in folate deplete men, several factors with roles in one‐carbon metabolism interact with the MTHFR C677T genotype to affect plasma tHcy.