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Effects of P deficiency on growth and tissue specific stem cell proliferation in neonatal pigs
Author(s) -
Alexander Lindsey S,
Mahajan Avanika,
Flann Kyle L.,
Rhoads Robert P.,
Odle Jack,
Stahl Chad H.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.33.4
Subject(s) - endocrinology , medicine , mesenchymal stem cell , cell growth , stem cell , biology , bone growth , chemistry , andrology , biochemistry , microbiology and biotechnology
Dietary phosphorus (P) deficiency reduces both muscle and bone growth in growing pigs. While satellite cells and mesenchymal stem cells are essential to postnatal muscle and bone growth, respectively, no work has investigated the possible effects of P nutrition on the proliferation of these cells. To determine the effect of dietary P deficiency during early‐postnatal growth, twenty 1‐day old pigs were fed either a P adequate (PA) or 20% P deficient (PD) liquid diet for 15d. By Day 5 and throughout the trial, plasma P and PTH concentrations were reduced (P < 0.05) in PD fed pigs. At Day 5 the PD pigs also had higher (P < 0.05) plasma Ca concentrations; however this difference was no longer significant by Day 10. Despite similar feed intakes, pigs fed the PA diet gained approximately 80% more weight (P < 0.05) than their PD fed counterparts. Additionally, PA fed pigs had radial bones with greater (P < 0.05) fresh, dry, and ash weight than the PD fed pigs, however the percentage of bone mineral content was not altered based on diet. The PD fed pigs had reduced (P < 0.05) proliferation of both satellite cells isolated from the loin muscle and mesenchymal stem cells isolated from bone marrow. The reduction in satellite cell and mesenchymal stem cell proliferation in P deficient animals may help explain the mechanism by which dietary P deficiency negatively affects muscle and bone growth.