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Probing RNA sequence specificity and function of PUF proteins
Author(s) -
Hall Traci,
Miller Matthew,
Wang Yeming,
Zhu Deyu,
Opperman Laura,
Stumpf Craig,
Wickens Marvin
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.326.2
Subject(s) - rna binding protein , messenger rna , rna , translation (biology) , microbiology and biotechnology , biology , computational biology , sequence (biology) , function (biology) , genetics , gene
PUF proteins, named for Drosophila PUMILIO and C. elegans FBF (fem‐3 binding factor), bind specifically to their mRNA targets and decrease expression by promoting mRNA decay or repressing translation. RNA recognition is critical to direct regulatory machinery to the appropriate target mRNAs. We have used a combination of structural and biochemical methods to examine the sequence specificity of PUF proteins from S. cerevisiae and C. elegans. Such studies have allowed us to appreciate how PUF proteins have been adapted to bind specifically to RNA using conserved and protein‐specific features.