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Microvascular response to ischemia in mouse spinotrapezius muscle: lessons for human vascular variability
Author(s) -
Mac Gabhann Feilim,
Bailey Alexander M,
Skalak Thomas C,
Peirce Shayn M
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.304.3
Subject(s) - ischemia , arteriole , ligation , medicine , disease , biology , neuroscience , pathology , cardiology , microcirculation
One of the great promises of Systems Biology is the refinement of therapeutic strategies through identification of vulnerable or responsive patient subpopulations. Ultimately, this leads to personalized medicine: interpersonal variability can influence both the severity of disease and the therapies that will be effective for each individual. Inbred mouse strains allow us to reproducibly mimic some of these variable physiologies and pathologies. Other groups have previously identified the remodeling responses of mouse strains to ischemic ligation of hindlimb arteries. Here we induce arterial ischemia in the mouse spinotrapezius, a model system that allows for the whole‐mount, en‐face visualization of the entire muscle microvasculature. We show that mice can be divided into two groups: ischemia‐vulnerable, characterized by a dendritic arteriolar structure, and ischemia‐protected, characterized by arteriole‐to‐arteriole connections. These groups ‐ even from different strains ‐ consistently respond differently to ischemic insult, and we identify potential for therapeutic pre‐treatment that can create an ischemia‐protected vasculature. Support provided by: NIH‐ HL082838 ‐02 and NIH‐T32‐HL007284.