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The Role of Fibronectin During Vasculogenesis
Author(s) -
Rupp Paul A.,
Kosa Edina,
Szabo Andras,
Filla Mike B.,
Czirok Andras
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.299.3
Subject(s) - fibronectin , vasculogenesis , microbiology and biotechnology , endothelial stem cell , biology , quail , chemistry , extracellular matrix , biochemistry , stem cell , in vitro , endocrinology , progenitor cell
The role of fibronectin in vasculogenesis was studied with time‐lapse deconvolution microscopy in quail embryos and in cultures of endothelial cells invading 3D collagen I gels. We show that fibronectin accumulates very early at the endothelial cell surfaces ‐‐ sometimes earlier than the endothelial cell‐surface epitope QH1 is detectable. By analyzing the displacements of pulse‐labeled fibronectin fibers around the nascent vessels, we show that the fibronectin accumulated is of mesodermal origin, and additional filaments are not assembled de novo during the process. Correlation analysis of endothelial cell movements and the morphology of fibronectin filaments show that fibronectin does not act as a vascular pre‐pattern to guide multicellular sprouts. However, fibronectin remodeling is essential to normal vasculogenic sprout formation ‐‐ perturbed fibronectin assembly alters endothelial cell motility. The work was funded by NIH (R01 HL87136).