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Effects of Polyphenolics from Grape (Vitis rotundifolia) and acai (Euterpe oleracea Mart.) on the expresion of microRNAs relevant to inflammation in vascular diseases
Author(s) -
Noratto Giuliana Doris,
Angel Gabriela,
MertensTalcott Susanne U.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.230.3
Subject(s) - umbilical vein , polyphenol , inflammation , reactive oxygen species , chemistry , lipopolysaccharide , apoptosis , proinflammatory cytokine , tumor necrosis factor alpha , dichlorofluorescein , human umbilical vein endothelial cell , pharmacology , biochemistry , microbiology and biotechnology , biology , antioxidant , immunology , in vitro
The cardio‐protective and anti‐innflammatory effects of polyphenolics have been demonstrated. It was the objective to investigate the anti‐inflammatory effects of polyphenolics from grape (Gp) and açai (Ac) in human umbilical vein endothelial cells (HUVEC). Cells were treated with Ac or Gp phenolics (5‐20 mg/L) and inflammation was induced with glucose (Gluc) (25mM) or lipopolysaccharide (LPS) (1ng/mL). The generation of reactive oxygen species (ROS) was determined with the dichlorofluorescein assay. MicroRNAs and mRNAs were quantified by RT‐PCR. Ac and Gp phenolics (5‐40mg/L) did not inhibit cell proliferation and moreover protected cells against Gluc induced ROS (Ac: 21‐ 57% and Gp: 37‐ 40%). Ac and Gp reversed Gluc‐induced 7.2‐fold increased expression of mir‐146a down to control levels in a dose‐dependent manner. Additionally, Ac and Gp reduced the mRNA expression of tumor necrosis factor receptor I (TNFRI) (up to 66%) and interleukin (IL‐8) (up to 68%). Gp and Ac decreased LPS‐induced expression of ICAM (up to 40‐60%) and angiotensin II (AGII) (up to 15%), and correspondingly increased expression of mir‐126 and mir‐155 (up to 3.8 and 4.4 fold, respectively) which are known inhibitors of ICAM and AGII, respectively. Overall, the investigated microRNAs may play a key role in the underlying mechanisms of the anti‐inflammatory effects of Gp and Ac polyphenolics.