Premium
Oxidative Stress is a Mediator of Endoplasmic Reticulum Stress in Liver Cells
Author(s) -
Gentile Christopher L,
Flory Kale,
Wei Yuren,
Wang Dong,
Pagliassotti Michael J
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.228.5
Subject(s) - endoplasmic reticulum , unfolded protein response , taurine , oxidative stress , chemistry , endocrinology , medicine , antioxidant , albumin , hepatocyte , biochemistry , biology , amino acid , in vitro
Lipids, particularly saturated lipids, induce endoplasmic reticulum (ER) stress in liver cells, although the mechanisms by which they do so are unclear. In the present study, we examined whether administration of the antioxidants taurine or alpha‐tocopherol could reduce lipid‐induced ER stress in liver cells. Primary rat hepatocytes and H4IIE liver cells (n=3 for all conditions) were incubated in a control medium containing 8mM of glucose or the same medium supplemented with albumin‐bound, long‐chain saturated (palmitate) or unsaturated (oleate) fatty acids (250 and 500 uM; 6 or 16 hrs) with and without taurine (0.25‐1.00%) or alpha‐tocopherol (VitE; 200 uM). Oleate did not induce ER stress in either primary hepatocytes or H4IIE cells regardless of dose or incubation time. Conversely, palmitate increased multiple markers of ER stress in both cell types. Taurine reduced palmitate‐induced ER stress in a dose‐dependent manner, reaching a ~50% reduction at the highest (1%) dose. VitE reduced palmitate‐induced ER stress by approximately 50%. These data suggest that lipid‐induced ER stress in liver cells is mediated, in part, by oxidative stress.