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Effects of parathyroid hormone on porcine mesenchymal stem cell differentiation
Author(s) -
Mahajan Avanika,
Stahl Chad H.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.220.4
Subject(s) - runx2 , parathyroid hormone , alkaline phosphatase , endocrinology , medicine , mesenchymal stem cell , chemistry , osteoblast , cellular differentiation , cell growth , hormone , calcium , biology , microbiology and biotechnology , enzyme , biochemistry , gene , in vitro
Parathyroid hormone (PTH) effects on mesenchymal stem cell (MSC) differentiation are dependent on the timing of treatment and culture conditions. In this study, we have characterized the effects of either continuous (CONT) or intermittent (2h/day, INT) PTH treatment on porcine MSC (pMSC) differentiation in media designed to promote proliferation. After 6d of culture, proliferation and gene expression were analyzed and alkaline phosphatase (ALP) activity was determined. The expression of Runx2, ALP , PPARG, LPL and PTHR were increased (P < 0.05) with either PTH treatment. Either PTH treatment also significantly increased (P < 0.05) the percentage of ALP+ cells (10% and 25% for CONT and INT compared to the control, respectively), and decreased (P < 0.05) cell proliferation (20 and 25% compared to the control for CONT and INT, respectively). We have previously shown that 1,25(OH) 2 D 3 stimulates adipocytic differentiation of pMSC and wanted to examine the interaction of PTH with 1,25(OH) 2 D 3 treatment. The ability of PTH to stimulate osteoblastic differentiation was completely inhibited by co‐treatment with 1,25(OH) 2 D 3 as seen by decreased (P < 0.05) ALP+ cells and reduced (P < 0.05) expression of Runx2 , ALP and PTHR by greater than 2 fold. These data suggest that PTH stimulates differentiation of pMSC. Also, 1,25(OH) 2 D 3 inhibits the ability of PTH to stimulate MSC toward osteoblast differentiation.