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Effects of high vitamin A intake on adipose tissue vitamin A levels in mice with impaired capacity for hepatic vitamin A storage
Author(s) -
Yang Jian Zhang,
Wongsiriroj Nuttaporn,
D'Ambrosio Dia.,
Blaner William S.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.215.3
Subject(s) - medicine , endocrinology , vitamin , retinol , basal (medicine) , adipose tissue , vitamin a deficiency , retinyl palmitate , chemistry , biology , diabetes mellitus
Both lecithin:retinol acyltransferase‐ ( Lrat −/− ) and cellular retinol‐binding protein type I‐ ( CrbpI −/− ) deficient mice have impaired hepatic vitamin A storage capacity. To gain insight into relationships between hepatic and adipose vitamin A storage, Lrat −/− , CrbpI −/− and Lrat −/− /CrbpI −/− mice were maintained for 4 wks on the basal AIN‐93M diet or this diet supplemented with 25‐fold excess retinol. Liver and adipose retinol/retinyl ester levels were determined by HPLC. Regardless of diet, no retinyl esters were detected in livers of Lrat −/− and Lrat −/− /CrbpI −/− mice; whereas wild type and CrbpI −/− mice respectively had levels of 363±60 and 162±41 on the basal diet and 4272±828 and 763±267 nmol/g on the excess diet. Adipose tissue total retinol levels for Lrat −/− , CrbpI −/− , Lrat −/− /CrbpI −/− and wild type mice fed the basal diet were respectively 10.2±3.1, 9.9±1.4, 8.2±0.4 and 3.2±0.3 nmol/g. On the excess diet the levels for Lrat −/− , CrbpI −/− , Lrat −/− /CrbpI −/− and wild type mice were respectively 153.6±27.2, 137.7± 43.8, 145.9±16.6, and 34.9±4.7 nmol/g. Thus, impaired capacity to store vitamin A in liver increased adipose accumulation of vitamin A for both basal and excess diet groups.