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Stem cells and the lung
Author(s) -
Aliotta Jason M,
Pereira Mandy,
Lee David,
Johnson Kevin,
Dooner Gerri,
Dooner Mark,
DelTatto Michael,
Papa Elaine,
Liu Liansheng,
Colvin Gerald A,
Quesenberry Peter J
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.186.2
Subject(s) - lung , haematopoiesis , bone marrow , microbiology and biotechnology , cell , biology , context (archaeology) , transcriptome , stem cell , cell culture , gene expression , gene , chemistry , cancer research , immunology , medicine , biochemistry , genetics , paleontology
Investigators have shown that transplanted murine marrow cells contribute to the cellular component of various non‐hematopoietic organs, including the lung. The mechanism responsible for this remains unknown. We wished to determine if marrow cell phenotype is influenced by lung‐derived factors by co‐culturing (separated by a cell‐impermeable membrane) murine whole bone marrow (WBM) cells with lung cells. Co‐cultured WBM expressed high levels of lung cell‐specific genes and protein, which was further augmented by radiation injury to the lung. Cell‐free lung conditioned media (LCM) had a similar effect on co‐cultured WBM, suggesting that factors smaller than the membrane pore were responsible for these changes. Co‐cultured WBM treated with transcriptional blocking agents had lung cell‐specific gene expression that was either up regulated or down regulated, implying transfer of a transcriptional agent. Microvesicles (MV) containing lung‐derived RNA and protein were isolated from LCM and found to enter WBM in culture which subsequently expressed lung‐specific genes. These data suggest that MV transfer may be an important mediator of cellular communication in the context of marrow cell plasticity.