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Dietary curcumin and limonin suppress murine CD4 + T‐cell activation
Author(s) -
Kim Wooki,
Fan YangYi,
Smith Roger,
Patil Bhimanagouda S.,
Jayaprakasha G. K.,
McMurray David N.,
Chapkin Robert S.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.110.6
Subject(s) - limonin , curcumin , t cell , stimulation , chemistry , inflammation , cd3 , microbiology and biotechnology , corn oil , pharmacology , biology , antigen , biochemistry , immune system , endocrinology , immunology , cd8 , food science
Phytochemicals may reduce chronic inflammation and cancer risk, in part, by modulating T‐cell nuclear factor kappa B (NF‐κB) activation. Therefore, we examined the effects of curcumin (C) and limonin (L) feeding on the NF‐κB pathway in CD4 + T‐cells. DO11.10 transgenic mice were fed diets containing 1% curcumin or 0.02% limonin, in the presence of n‐6 PUFA (corn oil) or with n‐3 PUFA (fish oil) for two weeks, followed by splenic CD4 + T‐cell isolation and stimulation with OVA 323‐339 peptide and antigen presenting cells from mice fed the conventional rodent chow. Both C and L diets suppressed (P<0.05) NF‐κB p65 nuclear translocation in activated CD4 + T‐cells. In contrast, AP‐1 c‐Jun and NFATc1 were not affected relative to control (corn oil, no C or L). CD4 + T‐cell proliferation in response to either mitogenic anti‐CD3/28 mAbs or antigenic stimulation by OVA peptide was also suppressed (P<0.05) by C as assessed by CFSE staining. In contrast, IL‐2 production was not directly associated with NF‐κB status. Interestingly, dietary combination with fish oil enhanced the suppressive effects (P<0.05) of C or L with respect to CD4 + T‐cell proliferation in response to anti‐CD3/28 mAbs. These results suggest that combination chemotherapy (fish oil + curcumin or limonin) may favorably modulate CD4 + T‐cell mediated inflammation. (Supported by NIH DK71707, CA59034, CA129444, P30ES09106, and USDA 2006‐34402‐17121)