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Selected trace metals inhibit transepithelial non‐heme iron transport by inhibiting the basolateral iron exit in human intestinal cells.
Author(s) -
Kim EunYoung,
Ham SooKyung,
Hao Sijie,
Han Okhee
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.105.1
Subject(s) - chemistry , absorption (acoustics) , caco 2 , inorganic chemistry , biochemistry , nuclear chemistry , biophysics , biology , cell , materials science , composite material
The relationship between iron and other trace metals has been recognized for many years. Intestinal absorption of other trace metals (e.g., Cd, Co, Zn and Mn) was significantly increased in iron deficient mice. On the other hand, other trace metals inhibit intestinal iron absorption. However, the mechanism by which these minerals interact in the absorption process is unknown. To investigate the interactions between iron and other trace metals in the process of intestinal absorption, we examined effects of selected trace minerals on the basolateral iron transport and apical iron uptake in fully differentiated Caco‐2 cells grown on microporouse membrane inserts. The addition of equimolar concentration of the selected trace metals (Cd, Co, Cu, Mn and Zn) significantly ( P <0.05) inhibited the basolateral 55 Fe transport. To examine if these metals decrease the basolateral iron transport by inhibiting the apical iron uptake, we next assessed effects of these minerals on the apical iron uptake. While the addition of Cu significantly (P<0.05) inhibited the apical 55 Fe uptake, Cd, Mn and Zn did not influence the apical 55 Fe import. Addition of equimolar concentration of Co significantly (P<0.05) increased the apical 55 Fe uptake. These results suggest that some selected trace metals inhibit non‐heme iron absorption by decreasing the basolateral iron export rather than the apical iron uptake in intestinal cells. (Supported by NIH R21DK069946 and USDA: 2005‐35200‐16543.)