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Overexpression of adipogenin enhances the fat accumulation of C2C12 myoblasts
Author(s) -
Song SangHoun,
Roh SangGun,
Katoh Kazuo
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1039.3
Subject(s) - c2c12 , adipogenesis , adipocyte , oil red o , adipose tissue , transfection , microbiology and biotechnology , cellular differentiation , leptin , chemistry , biology , lipid droplet , endocrinology , myocyte , gene , myogenesis , biochemistry , obesity
Adipogenin is known that its expression is higher in adipose tissue and increases during the adipocyte differentiation. However, the roles and mechanisms of adipogenin on fat accumulation and adipocyte differentiation remain largely unknown. In this study, we investigated whether constitutive overexpression of adipogenin enhances adipogenic differentiation and fat accumulation in C2C12 cells. Several clones expressing high levels of adipogenin were isolated and characterized. Cloned cells were proliferated to confluent, and differentiated with adipogenic differentiation medium. After induction of differentiation, cells were stained with Oil Red O and mRNA expression was measured at day 5. Fat accumulation was observed in adipogenin‐transfected C2C12 cells at 2 day after induction of differentiation and the lipid drops were larger at day 5. The expression of adipogenic maker genes, PPAR‐γ2 and leptin, was detected in cells transfected with adipogenin. These results demonstrate that overexpression of adipogenin in C2C12 enhances fat accumulation in vitro.