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Diurnal rhythms of muscarinic receptor expression in the eye of zebrafish
Author(s) -
Capalbo Elizabeth Louise,
Garcia Dana M
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1033.4
Subject(s) - muscarinic acetylcholine receptor , muscarinic acetylcholine receptor m1 , receptor , microbiology and biotechnology , zebrafish , biology , muscarinic acetylcholine receptor m3 , muscarinic acetylcholine receptor m4 , chemistry , biochemistry , gene
In teleosts, pigment granules disperse into the apical projections of the RPE to protect photoreceptors from photobleaching in ambient daylight. Activation of muscarinic receptors induces light‐adaptive pigment granule dispersion in isolated RPE. In order to determine whether muscarinic receptors could be involved in acetylcholine‐mediated pigment granule dispersion in vivo, we looked at muscarinic receptor expression in zebrafish eyes at time points in which the pigment granules of the RPE are fully dispersed or fully aggregated, midday and midnight, respectively. We used qRT‐PCR to study the differential expression of muscarinic receptors. Our data indicate that M3 muscarinic receptor RNA is expressed at higher levels at midnight than midday. Studies are underway to determine whether other subtypes of muscarinic receptors are also differentially expressed and to localize expression of the receptors in retinal sections using in situ hybridization and immunofluorescence microscopy. This work was supported by NSF IOB 0615762.