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Insulin Therapy Effects on Bone Health in Rats Following Severe Burn and Disuse
Author(s) -
Baer Lisa A,
Pidcoke Heather F,
Wu Xiaowu,
Silliman David T,
Walters Thomas,
Tou Janet,
Wolf Steven E,
Wade Charles E
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1031.1
Subject(s) - medicine , endocrinology , bone mineral , insulin , hindlimb , saline , femur , lean body mass , diabetes mellitus , burn injury , body weight , surgery , osteoporosis
Burn with hindlimb unloading (BH) results in substantial and sustained bone loss in rodents. We observed decreases in bone mineral density (BMD), content (BMC), and strength using this model. Insulin used for glucose control in burns alters burn induced hypermetabolic responses. The present study was conducted to determine if daily insulin treatment could offset effects of BH on body mass, bone mass and morphology. Male rats were randomized into two groups: BH‐Vehicle (BHV) or Insulin‐Treated (BHI). Rats received a 40% total body surface area scald burn and following recovery immediately hindlimb unloaded. Daily subcutaneous injections were either long‐acting insulin or 0.9% saline. Fourteen days after injury body mass, visceral fat, femur weight and length were measured, and BMD and BMC were determined by DEXA. Body mass and fat were significantly heavier in BHI. No differences were observed in femur weight, length or BMD, however BHI showed a significant decrease in BMC. Even though insulin sustained body mass and fat, there were minimal effects on bone. Insulin therapy may not be effective in maintaining bone health in burn patients. US Army MRMC & Juvenile Diabetes Foundation