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Amniotic fluid stem cells: growth, characterization and differentiation
Author(s) -
Janz Felipe Lara,
Debes Adriana,
Duarte Sergio,
Cavaglieri Rita,
Moron Antonio Fernandes,
Zugaib Marcelo,
Bydlowski Sergio Paulo
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1030.4
Subject(s) - homeobox protein nanog , mesenchymal stem cell , adipogenesis , stem cell , microbiology and biotechnology , flow cytometry , cd44 , cellular differentiation , multipotent stem cell , amniotic stem cells , induced pluripotent stem cell , chemistry , biology , oil red o , chondrogenesis , adult stem cell , embryonic stem cell , cell , biochemistry , gene , progenitor cell
Amniotic fluid (AF) present different cellular types in its composition and a specific group of these cells shows multipotent characteristics as high proliferation ratio and increased differentiation ability. The aim of our study is to better characterize these cells and evaluate their plasticity. AF cells were investigated regarding the expression of undifferentiation markers (Oct‐4, Sox‐2, Nanog) by RT‐PCR, surface protein identification by flow cytometry and differentiation assays into adipogenic, chondrogenic and osteogenic lineage, confirmed with Alizarin Red, H&E and Oil Red O stains respectively. AF cells showed high proliferation ratios and expressive doubling time. They also expressed pluripotent stem cell‐specific genes (Oct‐4, Sox‐2, Nanog) and were positive for mesenchymal membrane markers (CD29, CD44, CD133). They were also capable to differentiate into distinct lineages: adipocytes, osteocytes and chondrocytes. In conclusion, AF is a promising source of mesenchymal stem cells with high differentiation potential. Research governmental support: by CNPq.