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Human serum as an alternative for amniotic fluid stem cells culture media for clinical use
Author(s) -
Duarte Sergio,
Debes Adriana de Aguiar,
Cavaglieri Rita de Cássia,
Janz Felipe de Lara,
Lopes Marco Antonio Borges,
Bydlowski Sérgio Paulo,
Zugaib Marcelo
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1030.1
Subject(s) - homeobox protein nanog , sox2 , cd90 , stem cell , mesenchymal stem cell , amniotic fluid , embryonic stem cell , cd44 , andrology , cd34 , microbiology and biotechnology , biology , amniotic stem cells , immunology , induced pluripotent stem cell , adult stem cell , medicine , cell , fetus , genetics , pregnancy , gene
Objective to examine growth, phenotypic characteristics, gene expression and differentiation of amniotic fluid stem cells (AFSC) using human serum in culture media. Introduction AFSC are promising candidates for cell‐based therapies. They are obtained by prenatal screening, without raising the ethical concerns associated with human embryonic research. One major obstacle for their clinical use is the biosafety of fetal calf serum (FCS). Methods Amniotic fluid cells obtained from 2nd trimester amniocentesis were grown in α‐MEM+human serum20%. Attached cells were cultured and counted under microscope, investigated about the expression of Oct‐4, SOX2 and Nanog, by RT‐PCR and about phenotype by FACS‐analysis. Induced osteogenic and chondrogenic differentiation were confirmed with Alizarine Red and H&E stains respectively. Results AFSC showed high proliferation ratios; expressed Oct‐4, SOX2 and Nanog; stained positive for CD29, CD44, CD90, CD105 and negative for CD14, CD31, CD34, CD45, CD106 and CD133; showed osteogenic and chondrogenic potential. Conclusions Growth of AFSC in a FCS‐free medium is feasible. Can be isolated easily, proliferate quickly, show a mesenchymal phenotype and have osteogenic and chondrogenic potential. Moreover, Oct‐4, SOX2 and Nanog, expressed by AFSC, are associated with the pluripotency and for the maintenance of stem cells.