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Analysis of arginase and nitric oxide synthase (NOS) to excess collagen deposition in inhalation injury
Author(s) -
Sousse Linda Elias,
Wells Sandra,
Enkhbaatar Perenlei,
Esechie Aimalohi,
Traber Daniel L
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1025.6
Subject(s) - arginase , inhalation , nitric oxide , smoke inhalation injury , medicine , smoke inhalation , burn injury , nitric oxide synthase , lung , arginine , endocrinology , total body surface area , edema , chemistry , anesthesia , biochemistry , surgery , amino acid
Approximately 15,000 United States victims of burn trauma have inhalation injuries and suffer from acute lung injury. Burn victims have decreased levels of arginine, which can contribute to excessive collagen synthesis. We hypothesize that lung arginase activity is increased by burn and inhalation injury in murine and ovine models, which contributes to collagen deposition. C57BL6 mice were divided into three groups (n=6): Control/Uninjured, pre‐injured, and injured mice with a burn and smoke injury. Female sheep were divided into the following groups (n=4): SHAM, Uninstrumented, and injured sheep with burn and smoke injury sacrificed after 4, 8, 12, 18, 24, and 96 hours and 2 weeks. Murine arginase activity (Control: 0.159 ± 0.004, Injured: 0.329 ± 0.076), MPO activity, NOx activity, and iNOS activity (p < 0.0001) significantly increased after the injury, and immunostaining showed septal thickening and edema. Arginase activity increased directly in sheep after increasing time after the injury. The increase in arginase activity could lower body arginine levels and lead to the uncoupling of NOS and the increase in asymmetrical dimethyl arginine formation. The increase in arginase is may be vital to the increase in collagen deposition and may interfere with gas exchange in the lung. Supported by the Shrine Hospital for Children Grant No. 8541