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Effect of G‐CSF on pulmonary emphysema
Author(s) -
Ito Hiromichi,
Matsushita Shonosuke,
Ishikawa Shigemi,
Sakakibara Yuzuru
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1024.1
Subject(s) - elastase , granulocyte colony stimulating factor , medicine , lung , progenitor cell , bone marrow , alveolar wall , pathology , van gieson's stain , peripheral , endocrinology , andrology , chemistry , stem cell , h&e stain , immunohistochemistry , biology , biochemistry , chemotherapy , genetics , enzyme
Purpose G‐CSF is a cytokine to stimulate the bone marrow to produce granulocytes and stem cells. Recently it has demonstrated that G‐CSF mobilize the endothelial progenitor cell (EPC) to peripheral circulation and pluripotency of EPC for tissue repair, we investigated the effect of G‐CSF on pulmonary emphysema (PE). Methods Male Wistar Rat (6 wks, n=18) were divided into two groups; Control (PE; n=9) group and G‐CSF group (PE treated with G‐CSF; n=9). PE was induced by the injection of porcine elastase (200 U/kg) through trachea. G‐CSF was injected subcutaneously (100 microgram/kg/day, 12 days). Number of EPCs in peripheral blood was calculated at 13 days after initiation of G‐CSF injection by flow cytometry. Seven weeks after from injection of elastase, rats were sacrificed and evaluated by histopathology (HE and Elastica Van Gieson stain). PE was evaluated by the number of crossing alveolar wall in lattice with 100 micrometer. Results EPC was significantly increased by the use of G‐CSF (G‐CSF 4822 vs. Control 2814 per micro liter, p<0.05). The number of crossing alveolar wall is significantly preserved in G‐CSF treated rats compared with non‐treated rats (G‐CSF 2.660.29 vs. Control 1.660.33, p<0.0005). Conclusion G‐CSF preserved the alveolar structure in pulmonary emphysema significantly at least in part due to increase of endothelial progenitor cells in peripheral circulation.