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Net and Unidirectional Liquid Fluxes Across Porcine Bronchial Airways in Health and During Bacterial Pneumonia
Author(s) -
Martens Chelsea J.,
Schuler A. Michele,
Ballard Stephen T.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1023.7
Subject(s) - airway , absorption (acoustics) , mucociliary clearance , secretion , pneumonia , chemistry , respiratory system , medicine , materials science , lung , anesthesia , composite material
The epithelium of the tracheobronchial airways is capable of both active absorption and secretion of ions and liquid to maintain adequate volumes of airway surface liquid to support mucociliary transport and prevent airway obstruction. Using cannulated, liquid‐filled bronchi from pigs, rates of liquid flux across the airway wall were measured in the presence and absence of NPPB (an anion channel blocker that inhibits liquid secretion). Net and unidirectional liquid fluxes were determined. Responses from healthy animals were compared to animals diagnosed with bacterial pneumonia (Pasteurella multocida and Streptococcus suis). Unidirectional liquid secretion in infected airways (2.35± 0.55 μl/cm 2 /h) was significantly (p<.05) greater than in healthy airways (0.40±0.66 μl/cm 2 /h). Unidirectional liquid absorption in infected airways (3.29±0.47 μl/cm 2 /h) was reduced relative to healthy airways (4.65±0.55 μl/cm 2 /h), but this difference was not significant. In healthy airways, the net liquid flux was absorptive (4.25±0.62 μl/cm 2 /h). In the infected airways, the net liquid flux was significantly reduced (0.93±0.43 μl/cm 2 /h). We conclude that the bronchial airways of animals infected with these pathogens exhibit a net reduction in absorption of airway fluid that results from both reduced unidirectional absorption and augmented unidirectional secretion. (Support: NIH 063302, CFF BALLAR07XX0)