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Changes in ECM proteins, decorin and biglycan, during adipogenesis in 3T3‐L1 cells and in adipose tissue of mice on a high fat diet
Author(s) -
Ajuwon Kolapo M,
Cruz Meliza,
Buhman Kimberly
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1022.12
Subject(s) - biglycan , adipogenesis , decorin , adipose tissue , adipocyte , extracellular matrix , microbiology and biotechnology , medicine , endocrinology , white adipose tissue , chemistry , biology , proteoglycan
Adipogenic conversion requires an extensive remodeling of the extracellular matrix (ECM) to allow for adipocyte hypertrophy, hyperplasia and neovascularization of the tissue. Although ECM remodeling is known to be important in adipogenesis, details of the mechanisms and how this process determines the overall fate of the adipocyte and its functionality are poorly understood. However, it is known that the ECM plays significant role in obesity, inflammation and insulin resistance. We have examined the expression of 2 proteoglycans, decorin and biglycan, during adipogenic conversion of 3T3‐L1 cells and in obesity. Both decorin and biglycan are highly expressed in confluent adipocytes. However, after induction of adipogenesis, the mRNA expression of both proteoglycans falls precipitously to about 10% of the level at confluence. This reduced level of expression was noticeable 2 days after adipogenic induction and was sustained until day 4, after which the expression level begins to rise. Nevertheless, the expression at day 12 remains at approximately 50% of the level at confluence. Expression of both proteoglycans was also significantly induced in the epididymal adipose tissue of mice on a high fat diet compared to those on regular chow. Because both decorin and biglycan are differentially regulated during adipogenesis, they could play significant regulatory roles in adipogenesis and obesity.