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Effects of high salt diet on NF‐KB and CREB DNA binding activity in heart, kidney and hypothalamus of SHR
Author(s) -
Shang Qianhui,
Wang HongWei,
Stewart Alexandre FR,
Leenen Frans H. H.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1017.17
Subject(s) - creb , endocrinology , medicine , kidney , chemistry , hypothalamus , transcription factor , biochemistry , gene
In Dahl S or SHR on high salt diet, activation of NF‐κB contributes to cardiac hypertrophy and renal damage, which may be offset by activation of CREB. The brain RAAS mediates the salt‐induced hypertension, possibly by NF‐κB in the PVN. We assessed effects of high salt diet on DNA‐binding activity of NF‐κB and CREB in the heart, kidney and PVN of WKY rats and SHR on high (8%) or reg (0.6%) salt diet for 2 or 4 weeks (n=6). NF‐κB and CREB DNA binding activity were studied by electrophoretic mobility shift assay. In WKY, high salt only increased CREB DNA binding activity in the LV and NFκB in the PVN. In SHR, high salt increased NF‐κB DNA‐binding activity in the heart and kidney, but decreased CREB binding activity in the heart, kidney, and the PVN. Conclusion High salt causes an inverse relationship between NF‐κB and CREB in the LV, kidney and PVN which may contribute to salt induced sympathetic hyperactivity, LV and renal hypertrophy in SHR.