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High fat diet does not affect ADMA levels or ADMA regulatory enzymes in female Borderline Hypertensive Rats (BHR)
Author(s) -
White Leslie Dallas,
Mitra Anaya,
Sasser Jennifer M,
Wagner Laszlo,
Rowland Neil E.,
Baylis Chris
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1014.1
Subject(s) - medicine , endocrinology , enos , asymmetric dimethylarginine , offspring , western blot , chemistry , arginine , biology , nitric oxide , nitric oxide synthase , pregnancy , biochemistry , amino acid , gene , genetics
In this study, we tested the hypothesis that a HF diet increases ADMA levels in female BHR. Litters from male spontaneously hypertensive rats crossed with female Wistar rats that were maintained on either a HF diet (60% fat) or low fat diet (LF, 10% fat) for 6‐8 weeks prior to mating were used in this study. Offspring were divided into two groups: HHH (gestated in and suckled by a HF dam and weaned onto a "junk food" diet; n=6) and LLL (gestated in and suckled by a LF dam and weaned onto a standard chow diet; n=6). Rats were maintained on the respective diet for over 10 months, and then plasma levels of ADMA were measured by HPLC. HF diet increased body weight (499±32 vs 412±19 g, p<0.05 vs LF) but had no effect on blood pressure (118±7 vs 128±4, anesthetized direct arterial measurement). Analysis showed no significant changes in plasma ADMA levels (HHH: 0.18±0.02, LLL: 0.19±0.01 μmol/L). Kidney cortex abundance of the ADMA‐generating and degrading enzymes, protein methyltransferase‐1 (PRMT‐1) and dimethylarginine dimethylaminohydrolase (DDAH), respectively, and endothelial NOS (eNOS) were measured by Western blot. No differences in protein expression for any of the proteins were observed between groups (Table). Female BHR fed a HF diet did not express changes in plasma ADMA levels or DDAH, PRMT‐1 and eNOS expression. (Western Blot data expressed as IOD/Ponceau red/Positive Control ± SEM)