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Age dependent protein expression of renal acid‐base transporters in rats with congenital ureter obstruction
Author(s) -
Wang Guixian,
Topcu Sukru Oguzkan,
Djurhuus Jens Christian,
Nielsen Soren,
Frokiaer Jorgen
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1012.10
Subject(s) - pendrin , kidney , medicine , endocrinology , transporter , urinary system , urine , acid–base homeostasis , excretion , immunocytochemistry , ureter , chemistry , urology , biochemistry , gene
To clarify the molecular mechanisms of urinary acidification defects in response to congenital partial unilateral ureteral obstruction (PUUO) new born male Wistar rats were subjected to PUUO within the first 24‐48 hours of life to mimic the congenital ureteral obstruction, and were observed for 7 and 14 weeks. PUUO rats were challenged by acid loading with NH 4 Cl given by gavage for 2 days before termination. Semiquantitative immunoblotting revealed that the abundance of renal acid‐base transporters NHE3, NBC1, NBCn1, pendrin and Na + ‐K + ‐ATPase were increased in both obstructed and non‐obstructed kidneys 7 weeks after induction of neonatal PUUO. This was confirmed by immunocytochemistry. In contrast, 14 weeks of PUUO caused a reduction in the abundance of NBC1, NBCn1 and Na + ‐K + ‐ATPase in the obstructed kidney. Consistent with this the plasma pH and HCO 3 could maintain normal in PUUO 7 week rats, but dramatically reduced in PUUO 14 week rats under the acid loading. These time/age dependent changes in protein expression were associated with parallel changes in the renal ability to acidify urine in response to acid loading. In conclusion the study demonstrated that 1) the reduction protein abundance of renal acid‐base transporters is time/age dependent in response to PUUO and 2) impairment of urine acidification develops gradually in parallel with the changes in protein expression in response to partial obstruction induced neonatally.