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Temporal genomic transcriptional profiling of DSS induced colitis by microarray
Author(s) -
Fang Kai,
Bruce Megan,
Kevil Christopher G.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1006.12
Subject(s) - colitis , inflammation , angiogenesis , gene expression , microarray , gene expression profiling , inflammatory bowel disease , biology , gene , microarray analysis techniques , cancer research , fold change , immunology , microbiology and biotechnology , medicine , genetics , disease
We have previously reported that the dextran sodium sulfate (DSS) induced colitis model involves a complex relationship between inflammation and angiogenic activity in that inflammation and angiogenesis serve to perpetuate chronic inflammation. Here we performed genome transcription profile analysis of 3% DSS induced colitis using the Affymetrix mouse genome 430 2.0 Array from colon tissues taken at day 0 (D0), 2 (D2), 4 (D4), and 6 (D6). Several inflammatory genes were greatly up‐regulated, such as Interleukin‐6 (0.9, 8.6 and 80.7 fold increase at D2, D4, and D6), Mmp 10 (1, 3, 22 fold increase at D2, D4, and D6), and Mmp 13 (2 and 21 fold increase at D4 and D6). Angiogenesis related genes were also significantly up‐regulated, such as HGF (0.4 and 2.8 fold increase at D4 and D6), Map3k8 (3 fold increase at D6), and Epiregulin (3.7 fold increase at D6). Increased inflammatory and angiogenic gene expression was observed in a proportional manner with earlier changes in inflammatory gene expression. Several genes were also significantly down regulated such as Trpm6 (38%, 43% and 71% at D2, D4 and D6), Repin1 (16%, 54%, and 76% at D2, D4 and D6), and Pnpla6 (41%, 59% and 56% at D2, D4 and D6). Our study demonstrates that inflammatory and angiogenic activities are linked during experimental colitis and delineate distinct time points of genome transcriptional change which may suggest new targets for the treatment of colitis.