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The Influence of Tungstate‐Exposure on Immunological Response to RSV Infection in C57BL/6 Mice
Author(s) -
Fastje Cynthia Darlene,
Harper Kevin,
Park Yong Soo,
Wong Simon S.,
Witten Mark L.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1003.8
Subject(s) - immunology , sodium tungstate , transcriptome , microarray , gene , biology , microbiology and biotechnology , saliva , gene expression , chemistry , genetics , biochemistry , tungsten , organic chemistry
Significantly elevated concentrations of sodium tungstate have been demonstrated in the atmospheric particulate matter in multiple communities experiencing elevated rates of childhood leukemia, but not in their respective control communities. Prenatal exposure to sodium tungstate in C57BL/6 mice significantly decreased the transcriptome expression of Dmbt1, a protein which functions to aggregate bacteria and viruses in lung mucosa and saliva. Additionally, network analysis of gene microarray data produced a significant network associated with hematological/immunological disease focused on genes involved in viral defense. We hypothesize that exposure to sodium tungstate alters immunological and hematological response to viral infection as measured by qRT‐PCR of RSV genes F & G during the fastigium of the infection, CBC with a differential, and RT 2 ‐PCR for genes associated with immunological function. C57BL/6 mice, exposed prenatally to sodium tungstate, demonstated a differential response to RSV viral infection. Additionally, tungsten exposure in male mice has a deleterious effect on testes function. Supported by EPA Grant EM‐96963201