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Deciphering the indolence of osteomyelitis: Evaluation of the uptake of Staphyloccocus aureus and silver nanoparticles into osteoblasts
Author(s) -
Wagner William,
Benjamin Joseph C.,
Webb Lawrence X.,
Coldren Faith,
Kute Timothy,
Martin Eileen,
Smith Thomas L.,
Smith Beth P.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1003.3
Subject(s) - staphylococcus aureus , flow cytometry , cytotoxicity , silver nanoparticle , chemistry , confocal microscopy , osteoblast , fluorescence microscope , microbiology and biotechnology , confocal , osteomyelitis , nanoparticle , fluorescence , bacteria , biophysics , biochemistry , nanotechnology , medicine , in vitro , biology , immunology , materials science , genetics , physics , geometry , mathematics , quantum mechanics
Chronic osteomyelitis caused by Staphylococcus aureus is difficult to treat because the bacteria persists inside osteoblasts. The first aim of this study was to assess the uptake potential of S. aureus into osteoblasts. Because novel therapies are needed to combat the increasing antibiotic resistance of S. aureus, the uptake of silver nanoparticles (a possible therapy) into osteoblasts also was examined. Osteoblasts were incubated with carboxyfluorescein‐succinimidyl ester (CFSE) labeled S. aureus or silver nanoparticles and stained for confocal microscopy. Incubated osteoblasts were evaluated using flow cytometry for their FITC fluorescence profile ( S. aureus) or SSC (side scatter) changes (nanoparticles). Osteoblasts were incubated with nanoparticles at different concentrations to determine their cytotoxicity. Flow cytometry showed that the FITC fluoresence was higher in infected osteoblasts due to incorporation of CFSE‐labelled S. aureus while SSC was greater due to the incorporation of silver nanoparticles. Confocal microscopy images confirmed the uptake of both S. aureus and nanoparticles. Nanoparticles demonstrated minimal cytotoxicity at low concentrations (MTS assay). Aided by a grant form the Orthopaedic Research and Education Foundation and the Translational Science Institute at Wake Forest University Health Sciences