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Angiotensin (1‐7) downregulation of AQP1 in rat proximal cell is mediated by ACE and ACE2 balance
Author(s) -
Palomino Zaira,
Casarini Dulce Elena,
Jung Flavia F
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1001.5
Subject(s) - bradykinin , angiotensin converting enzyme 2 , chemistry , angiotensin ii , stimulation , endocrinology , renin–angiotensin system , medicine , downregulation and upregulation , prostaglandin e2 , enzyme , prostaglandin , angiotensin converting enzyme , biology , biochemistry , receptor , covid-19 , blood pressure , disease , gene , infectious disease (medical specialty)
Aquaporin 1 (AQP1) is the major constitutively expressed water channel in the renal proximal tubule and thin descending limb where nearly 80% of water is reabsorbed. However, the factors regulating its expression are still elusive, we have shown that Angiotensin (1‐7) [Ang (1‐7)] downregulates AQP‐1 expression in immortalized rat proximal tubule cells (IRPTC). In this study, we investigated Bradykinin (Bk), Prostaglandin E2 (PGE2), Angiotensin Converting Enzyme I (ACE) and Angiotensin Converting Enzyme 2 (ACE2) as possible mediators of Ang (1‐7) effect on AQP1. After the time points stimulation release of Bk and PGE2 induced by stimulation with Ang‐(1‐7) was measure by HPLC and EIA respectivelly. There was a significant elevation of Bk (1.53 vs 0.43 nM/mg) and PGE2 (169.1 vs. control 23.3 pg/mL) release. Enzymatic activity showed that Ang (1‐7) inhibited the enzymatic activity of ACE ( control:0.46 ± 0.02 vs 5min: 0.04 ± 0.001 nM/min/mg) , and stimulated ACE2 activity (control: 180 ± 33.88 vs. 120 min: 1041.9 ± 77.2 nM/min/mg). This study demonstrates that the direct effect of Ang‐(1‐7) is mediated by release of BK and PGE2, as well as, inhibition of ACE and stimulation of ACE2.