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Automated planar patch clamp: Multiple applications for miniaturized devices
Author(s) -
Haarmann Claudia,
Haythornthwaite Alison,
Brueggemann Andrea,
Stoelzle Sonja,
Farre Cecilia,
George Michael,
Fertig Niels
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.1000.1
Subject(s) - patch clamp , shaker , planar , materials science , ion channel , electrophysiology , biomedical engineering , chemistry , optoelectronics , computer science , acoustics , engineering , physics , neuroscience , receptor , biochemistry , vibration , biology , computer graphics (images)
The Port‐a‐Patch is a miniaturized patch clamp rig which uses a planar borosilicate glass chip for performing electrophysiological recordings. Suction applied from the underside of the chip is used to attract a single cell to the recording site without the need for optical visualization. The Port‐a‐Patch has been successfully used for whole cell, perforated patch, and cell attached recordings of a variety of ion channels with high success rates (typically 60‐80%). Fast application of ligand is possible using the external perfusion system. Recordings from recombinant GABA A receptors (α 1β2γ2 ) expressed in HEK293 cells will be shown. Examples of voltage‐gated ion channels recorded using the Port‐a‐Patch will also be shown, including Na V 1.5 stably and Shaker K + transiently expressed in HEK293 cells. The effect of temperature on the amplitude and kinetics of hERG channels will also be presented. One of the newest and most exciting aspects of planar patch clamping using the Port‐a‐Patch is the ability to perfuse the internal membrane of the cell, whilst maintaining a continuous recording during the solution exchange. The internal perfusion system for the Port‐a‐Patch allows the exchange of up to 8 different solutions. Exemplar traces will be shown for regulation of TRP V1 by internal as well as external regulators. Such ease with which the internal solution may be exchanged is a unique feature to Nanion products.