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Resveratrol inhibits H2O2‐induced apoptosis in C2C12 myotubes
Author(s) -
Haramizu Satoshi,
Butler David C,
Asano Shinichi,
Alway Stephen E.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.94
Subject(s) - myogenesis , apoptosis , oxidative stress , chemistry , myocyte , dna fragmentation , microbiology and biotechnology , resveratrol , caspase 3 , c2c12 , biology , biochemistry , programmed cell death
Resveratrol (3,5,4′‐ trihydroxystilbene, RSV) is a natural polyphenol that is reported to have both anti‐apoptotic and pro‐apoptotic properties. The effects of RSV on oxidative stress‐induced apoptosis in skeletal muscle have not been well characterized. The purpose of this study was to determine if RSV inhibits induction of apoptosis by oxidative stress in myotubes. The C2C12 cells were cultured to 80% confluency and differentiated to form myotubes in low serum for 48 h. Myotubes were pretreated with RSV (0.01, 0.025, 0.05 mM) for 24 h then incubated in 1mM H 2 O 2 for 24 h. The myotubes were harvested for indices of oxidative stress and apoptosis including a DNA fragmentation apoptotic index, caspase activity, and apoptotic signaling proteins. The apoptotic index of myotubes pretreated with 0.025 mM and 0.05 mM RSV was significantly lower by 20% and 23%, respectively, than that in the group exposed to H 2 O 2 alone. The H 2 O 2 ‐induced increases in caspase 9, 3 and 8 were significantly inhibited by RSV, which coincided with higher Bcl‐2, catalase, GPx‐1 and phospho‐Akt proteins in cytoplasmic fraction. Furthermore, RSV inhibited AIF and Smac protein accumulation in the mitochondria‐free cytoplasmic protein fraction of H 2 O 2 treated myotubes. The data suggest that RSV can partially attenuate H 2 O 2 ‐induced apoptosis in C2C12 myotubes by inhibiting both intrinsic and extrinsic apoptotic signaling pathways.