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Adverse effects of maternal nutrient restriction on adipose tissue inflammation in juvenile offspring
Author(s) -
Budge Helen,
Gardner David S,
Sharkey Don,
Symonds Michael E
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.705
Subject(s) - offspring , adipose tissue , insulin resistance , endocrinology , medicine , cd68 , inflammation , pregnancy , adipose tissue macrophages , biology , obesity , metabolic syndrome , immunohistochemistry , genetics
Background: Maternal nutrient restriction (NR) during pregnancy can induce obesity and insulin resistance in the offspring but the mechanisms remain unclear. Increasing adiposity is associated with adipose tissue macrophage accumulation and inflammation predisposing to the metabolic syndrome. Aims: To determine the effects of maternal NR on the expression of key genes implicated in this process. Those juveniles exposed to late NR develop insulin resistance and increased adiposity (Gardner 2005). Methods: 17 singleton bearing sheep received either 100% metabolisable energy (ME) throughout pregnancy (controls), and 50% ME from days 0 to 30 (Early NR) or days 110 to 147 (Late NR). At 1 year perirenal adipose tissue (PAT) was analysed for the mRNA expression of CD68 (macrophage marker), toll‐like receptor 4 (TLR4) and IL‐6. Results: Weight at birth and 1 year were not different between the groups. Irrespective of maternal diet, TLR4 correlated with IL‐6 and CD68 (R 2 =0.92 and 0.69, P<0.0001). Conclusions: Maternal NR induces macrophage accumulation and inflammation within adipose tissue of offspring and in late NR results in insulin resistance and increased adiposity.