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The effect of short term higher versus lower fat intake on plasma triglycerides, VLDL‐TG fatty acid composition and hepatic fatty acid synthesis
Author(s) -
Wilke Michaelann S,
French Margaret A,
Goh Yeow K,
Ryan Edmond A,
Clandinin Michael Thomas
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.694
Subject(s) - chemistry , medicine , very low density lipoprotein , endocrinology , metabolism , triglyceride , palmitic acid , fatty acid , saturated fatty acid , lipid metabolism , cholesterol , biochemistry , biology , lipoprotein
Background: Low fat high carbohydrate (LF) diets increase plasma triglyceride (TG) levels, but the role of hepatic de novo fatty acid (DNFA) synthesis is uncertain. We previously found a relationship between TG level and DNFA synthesis. Objectives: To use isotopic methods to examine the effect of dietary fat on plasma TG FAs. We hypothesized that eucaloric higher fat (HF) intake would result in lower TG levels and VLDL‐TG saturated FA composition and DNFA synthesis changes. Methods: Six subjects were fed 2 diets differing in fat energy (LF<25%, HF>35%) for 3d (crossover, 1‐mo washout). Blood samples were drawn before and 24h after deuterium‐labeled water consumption. Results: Plasma and VLDL TG were lower following HF intake. Composition of VLDL‐TG FAs showed a higher amount of saturated FAs after LF intake. No significant change was found in total DNFA synthesis between diets, but LF resulted in more palmitic and stearic acid synthesis. A relationship was found between FA synthesis and plasma TG only for LF. Conclusion: When compared to LF, HF lowered plasma TG and resulted in important differences in VLDL‐TG FA composition that may have been influenced by DNFA synthesis. Funded by the CDA. Nutrition & Metabolism Biochemistry of Vitamins and Minerals (400‐ASN) Energy and Nutrient Metabolism (401‐ASN) Human and Clinical Nutrition (402‐ASN) Metabolic and Disease Processes (403‐ASN) Late Breaking Category ‐ Nutrition & Metabolism 400‐ASN Biochemistry of Vitamins and Minerals 401‐ASN Energy and Nutrient Metabolism 402‐ASN Human and Clinical Nutrition 403‐ASN Metabolic and Disease Processes