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Alterations of histone acetylations on the sucrase‐isomaltase and sodium‐glucose cotransporter 1 genes induced by dietary manipulations in mouse jejunum
Author(s) -
Goda Toshinao,
Honma Kazue,
Mochizuki Kazuki
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.691
Subject(s) - enhancer , histone h4 , acetylation , histone , biology , promoter , microbiology and biotechnology , chromatin immunoprecipitation , gene expression , biochemistry , chemistry , gene
We have previously reported that a diet rich in carbohydrate enhances the acetylations of histone H3 and H4 on the promoter or transcriptional region of mouse sucrase‐isomaltase (SI) gene and its mRNA levels in mouse jejunum. In this study, we found that the acetylation of histone H3 at lysine 9 was also enhanced on the promoter/enhancer region of the sodium‐glucose cotransporter 1 (SGLT1) gene in mice fed a diet rich in carbohydrate. Short‐term force‐feeding a diet containing fructose, but not glucose, caused an increase in SI mRNA levels within 6 h. Chromatin immunoprecipitation assay revealed that histones H3 and H4 on the promoter/enhancer regions of the SI gene were highly acetylated in mice given fructose, compared with those given glucose or water. These results suggest that SI and SGLT1 genes are coordinately regulated by alterations of histone acetylations on the promoter/enhancer region of the genes, and that the acute induction of SI gene expression by dietary fructose is also associated with acetylations of histones H3 and H4 on the promoter/enhancer regions of the SI gene.