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CD4 T cell help is required for developing appropriate CD8 recall responses to dominant minor histocompatibility antigen, H60
Author(s) -
Ryu Su Jeong,
Jung Kyoung Min,
Chang Jun,
Choi Eun Young
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.533
Subject(s) - minor histocompatibility antigen , cd8 , cytotoxic t cell , antigen , immunology , epitope , t cell , major histocompatibility complex , biology , immune system , transplantation , medicine , genetics , in vitro
Minor histocompatibility antigens (minor H Ags) are substantial impediments for the success of transplantation between MHC‐matched individuals. To investigate CD4‐help requirement for CD8 T cell responses to dominant minor H Ags, H60 was used as a model antigen, and CD8 T cell response to H60 was checked after immunizing female C57BL/6 (B6) mice with splenocytes from female (helpless condition) or male (with cognate CD4‐help) H60 congenic mice. Induction of primary H60‐specific CD8 T cell response was CD4‐help dependent. It was also required for the induction of the memory responses. The burst size of the memory response was reduced when female B6 mice, once helped during the primary response, were re‐challenged without CD4‐help. It was further reduced when the CD4‐help was not provided again at the third challenge. Presentation of CD4 and CD8 T cell epitopes by separate cells for the secondary response did not induce burst expansion of memory cells, but had the effect of extending the peak frequency for a long period of time. Our data suggest that the appropriate CD8 T cell responses for cellular antigen H60 depends on CD4‐help, and the best help is provided by cognate presentation of CD4 and CD8 T cell epitopes. The understanding of the nature of immune responses to dominant minor H Ags has implications in vaccine design for success in transplantation. (supported by KOSEF grant, R01‐2006‐10565‐0)