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Administration of rapamycin in vivo and in vitro prolonged allograft survival associated with iTregs
Author(s) -
Hou Guihua,
Han Jiankui,
Zheng Yongxian,
Zhang Chao,
Liang Ting,
Song Jing,
Wang Dan,
Xu Jia
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.529
Subject(s) - in vivo , foxp3 , in vitro , adoptive cell transfer , il 2 receptor , pharmacology , population , chemistry , immunology , medicine , biology , immune system , cytotoxic t cell , biochemistry , microbiology and biotechnology , environmental health
It has been reported that rapa could selectively expand the murine naturally occurring CD4+CD25+Foxp3+ Treg cells in vitro. However, the in vivo effect of rapa on the inducible CD4+CD25+Foxp3+ Treg cell population (iTregs) need to be elucidated. The aim of this study was to investigate the effect of rapa on activities of iTregs. The results indicated that rapa administration in vivo(14 consecutive days) significantly prolonged the allograft survival (13.2±1.5d to 22.8±2.1d).CD4+ T cells from tolerance mice treated with rapa in vitro result in obviously increase of iTreg, the higher expression of Foxp3 and IL‐10 secretion. Adoptive transfer of those CD4+ T cells obviously prolonged the allograft survival in allo‐transplanted SCID mice. This study indicated that rapa can expand the ratio of iTreg in vivo and in vitro in allografted model mice,and the expanded iTreg cells keep their inhibitory activities in vivo.