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Regulation of CD8+ Memory T‐Cell Differentiation by B Cells
Author(s) -
Nanjappa Som G,
Suresh M
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.510
Subject(s) - lymphocytic choriomeningitis , cytotoxic t cell , biology , cd8 , t cell , immunology , b cell , antigen , microbiology and biotechnology , antigen presenting cell , immune system , antibody , in vitro , genetics
Studies in B‐cell‐deficient mice have indicated that B cells might play an important role in regulating CD8+ T cell responses. The interpretation of experiments in B‐cell‐deficient mice is complicated due to the abnormal histological architecture of lymphoid organs. Additionally, the role of antigen‐specific B cells in regulation of CD8+ T cell responses has not been studied. The B‐cell transgenic mice (HEL‐ITg), in which all B cells express the antigen receptor for hen egg lysozyme, is an excellent model to study the role of antigen‐specific B cells in regulating CD8+ T cell responses without the complication of lymphoid abnormalities associated with B cell knockout mice. In response to an acute infection with lymphocytic choriomeningitis virus (LCMV), HEL‐ITg mice mounted a potent primary CD8+ T‐cell response. However, the functional and phenotypic maturation of memory CD8+ T cell was adversely affected in HEL‐ITg mice. LCMV‐specific memory CD8+ T cells were impaired in IL‐2 production and exhibited a predominantly CD62L lo /CD127 lo phenotype. Additionally, memory CD8+ T cells in HEL‐ITg mice provided poor protective immunity to secondary challenge. These studies show that antigenic‐specific B cells are essential for optimal CD8 T cell responses to an acute viral infection. Work was supported by a PHS grant to M. Suresh from NIH.