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Characterization of IL‐10 effects on antigen‐specific CD8 T lymphocytes during murine cytomegalovirus (MCMV) infection
Author(s) -
Gaddi Pamela Jean,
SalazarMather Thais P.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.509
Subject(s) - immunology , cd8 , cytotoxic t cell , biology , cytomegalovirus , spleen , immunity , t cell , cytokine , immune system , antigen , acquired immune system , virology , virus , herpesviridae , in vitro , viral disease , biochemistry
The mechanisms that determine the balance between antiviral inflammatory responses and prevention of overt pathology in the midst of these responses remain unclear. During murine cytomegalovirus (MCMV) infection, multiple antiviral and immunoregulatory cytokines are produced by cellular components of innate and adaptive immunity. The studies presented were conducted to characterize the effects of interleukin (IL)‐10 on CD8 T cell antiviral responses to acute MCMV infection. When compared to C57BL/6 control mice, our results demonstrate a significant increase in the proportions and absolute total numbers of antigen‐specific CD8 T cells in the spleens and livers of IL‐10‐deficient mice following MCMV infection. Furthermore, this accumulation was accompanied by an increase in the secretion of IFN‐γ from activated CD8 T cells. Additionally, we demonstrate that CD8 T cells contribute to the overall levels of IL‐10 in spleen and liver. Our studies also show that IL‐10‐deficiency does not enhance susceptibility to MCMV infection. Collectively, these studies establish an integral function for IL‐10 in modulating key CD8 T cell inflammatory and cytokine responses. This work is supported by the GAANN Training Grant and NIH R01CA‐102708. Pamela Gaddi is a Frederic Gorham Poole Endowed Fellow.