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Immunomodulatory potential of CVT‐E002 , a proprietary North American Ginseng extract, in an in vitro model of virus‐induced asthma exacerbation
Author(s) -
Adamko Darryl James,
Wu Yinqi,
Ilarraza Ramses,
Davoine Francis
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.479
Subject(s) - virus , cd86 , cd80 , immune system , immunology , in vitro , cytokine , il 2 receptor , medicine , virology , t cell , biology , cd40 , cytotoxic t cell , biochemistry
Rationale: Most asthma exacerbations are precipitated by airway virus infections. Understanding the inflammatory response to viruses could allow preventative immune modulating therapies. CVT‐E002 (commercially available as COLD fX ® ) is a patented poly‐furanosyl‐pyranosy‐saccharide‐rich extract of North American ginseng. Clinical trials show that CVT‐E002 prevents virus infection. Objectives: To determine whether CVT‐E002 has anti‐virus properties in vitro; and to understand its effects on human dendritic cells and lymphocytes. Methods: Human dendritic cells (DC) and T cells were cultured with or without virus/virus sham (RSV or RV). Flowcytometry and ELISA assays were performed. Results: RSV and RV upregulate CD25 expression in proliferating memory CD4+ T cells co‐cultured with DC (n=3). DC pretreated with CVT‐E002 induce increased CD25 on CD4+ T cells similar to virus infection alone. Combination of RV and CVT‐E002 augments this expression (n=5). DC with CVT‐E002 show increased expression of HLA‐DR, CD80, CD86 (n=2). T cells and DC incubated with either RSV or RV release IFN–γ, IFN‐α, TNF, and IL‐12 (n=1). CVT‐E002 pretreatment increases this release. Without virus, CVT‐E002 induces some cytokine release. Conclusion: CVT‐E002 appears to induce maturation of DC and induces increased T cell proliferation with increased antiviral cytokine responses. CVT‐E002 could be a useful immune modulator in the prevention of virus‐induced asthma exacerbation.