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Activated platelets enhance early neutrophil transmigration across IL‐1β stimulated endothelium
Author(s) -
Lam Fong,
Burns Alan,
Rumbaut Rolando
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.451
Subject(s) - platelet , umbilical vein , endothelium , adhesion , platelet activation , neutrophil extracellular traps , immunology , chemotaxis , inflammation , thrombin , protease activated receptor , neutrophile , chemistry , andrology , medicine , receptor , in vitro , biochemistry , organic chemistry
Background: Recent studies reveal platelets’ increasingly important role in inflammation in addition to thrombosis. Platelet‐neutrophil adhesive interactions with endothelium have been well described; however, whether platelets alter neutrophil migration across endothelium remains unknown. Hypothesis: Activated platelets enhance neutrophil transmigration across endothelial cells. Methods: Cultured human umbilical vein endothelial cells (HUVEC) were stimulated with IL‐1β 3–4 hours prior to the experiment. Neutrophils (PMN) and platelets isolated from healthy adults were injected into static adhesion chambers and recorded under phase‐contrast microscopy. Adhesion or transmigration of PMN alone, PMN with unactivated platelets, and PMN with platelets activated with thrombin receptor agonist peptide (TRAP) were compared using ANOVA. Results: At 500 seconds, PMN with TRAP‐activated platelets migrated significantly more than PMN alone (57±6% vs. 33±5%; p<0.01). PMN with unactivated platelets demonstrated a small increase in migration (40±4%; p<0.05) as compared to PMN alone. At 1000 seconds, there were no differences in either firm adhesion or transmigration of PMN between the groups. Conclusions: Under static conditions, activated platelets enhance early transmigration of PMN, but do not affect firm adhesion or late transmigration across IL‐1β‐stimulated HUVEC. Support: T32 HL07747‐15