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The LFA‐1 and ICAM‐1 adhesion pathway mediates the homeostatic migration of lymphocytes from peripheral tissues through lymphatic vessels into lymph nodes
Author(s) -
Xu Baohui,
Xu Zeyu,
Cook Rachel E,
Michie Sara A
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.439
Subject(s) - lymph , lymphatic system , pathology , peritoneal cavity , lymph node stromal cell , lung , lymphatic endothelium , biology , medicine , anatomy
The binding of LFA‐1 on lymphocytes to ICAM‐1/ICAM‐2 on endothelia is important for the migration of lymphocytes from blood vessels into lymph nodes. The roles of LFA‐1 and ICAM‐1 in the migration of lymphocytes from peripheral tissues through lymphatic vessels into lymph nodes have not been defined. In this study, we found constitutive expression of ICAM‐1 on lymphatic vessels in lymph nodes and uninflamed peripheral tissues, including lung, pancreas and skin, of wild type C57BL/6 mice. LFA‐1‐deficient lymphocytes transferred into blood vessels, skin, lung or peritoneal cavity of wild type mice migrated into the draining lymph nodes 70% less efficiently than did wild type lymphocytes. Wild type lymphocytes migrated from blood vessels into the lymph nodes of wild type and ICAM‐1‐deficient mice equally well. In contrast, wild type lymphocytes migrated from skin, lung or peritoneal cavity into lymph nodes of ICAM‐1‐deficient mice 60% less efficiently than into lymph nodes of wild type mice. As expected, memory lymphocytes migrated more efficiently to lymph nodes through lymphatic vessels than blood vessels. Taken together, these data indicate that LFA‐1/ICAM‐1 adhesion pathway is important in the homeostatic migration of lymphocytes, particularly memory T cells, into lymph nodes from peripheral tissues (Supported by NIH grant R01 DK67592).