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Human Lactoferrin attracts monocytes and activates monocyte‐derived Dendritic Cells to become mature antigen‐presenting cells
Author(s) -
De La Rosa Gonzalo,
Tewary Poonam,
Yang De,
Oppenheim Joost J
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.438
Subject(s) - lactoferrin , cd80 , immune system , cd86 , proinflammatory cytokine , dendritic cell , innate immune system , ovalbumin , monocyte , immunology , biology , microbiology and biotechnology , cd14 , antigen , chemistry , cd40 , t cell , cytotoxic t cell , inflammation , in vitro , biochemistry , genetics
Lactoferrin is an 80‐KDa iron‐binding protein present at high concentrations in milk and in the granules of neutrophils. It possesses multiple activities, including anti‐bacterial, anti‐viral, anti‐fungal, and even anti‐tumor effects. Most of its antimicrobial effects are due to direct interaction with pathogens, but a few reports show that it has direct interactions with cells of the immune system. Here we show the ability of recombinant human lactoferrin (Talactoferrin‐alpha, TLF) to chemoattract monocytes. What is more, addition of TLF to human peripheral blood or monocyte‐derived dendritic cell cultures resulted in cell maturation, as evidenced by upregulated expression of CD80, CD83, and CD86, production of proinflammatory cytokines, and increased capacity to stimulate the proliferation of allogeneic lymphocytes. When injected into mouse peritoneal cavity, lactoferrin also caused a marked recruitment of neutrophils and macrophages. Immunization of mice with ovalbumin in the presence of TLF promoted Th1‐polarized antigen‐specific immune responses. These results suggest that lactoferrin may play a role in the activation of both the innate and adaptive immune responses by promoting the recruitment of leukocytes and activation of dendritic cells.