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CXCR6 is required for T cell recruitment into injured gray matter in EAE
Author(s) -
Kim Jiyun Veronica,
Tadokoro Carlos E,
Shen Shiqian,
Jiang Ning,
Lafaille Juan J,
Dustin Michael L
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.424
Subject(s) - white matter , experimental autoimmune encephalomyelitis , spinal cord , foxp3 , multiple sclerosis , biology , immunology , chemistry , pathology , medicine , microbiology and biotechnology , neuroscience , magnetic resonance imaging , immune system , radiology
We examined the role of CXCR6 in entry of T cells into the white and gray matter of the CNS during experimental autoimmune encephalomyelitis (EAE) with and without a secondary injury to the cerebral cortex. CXCR6‐deficient mice were susceptible to EAE induced by MOG(35–55) peptide vaccination. At the peak of EAE, GFP + T cells were predominantly CD4 + , exclusively present in the spinal cord, and absent in the brain in both CXCR6 gfp /gfp and CXCR6 gft/+ mice. In the spinal cord, GFP + T cells were mostly restricted to the white matter with preferential rostral‐ caudal axis locomotion in both genotypes. During remission, IL‐17 and interferon‐γ producing T cells were GFP + , whereas Foxp3 + cells were 20% GFP + in both genotypes with no change in the ratio of Foxp3 + to IL‐17 producing T cells. Recruitment of GFP + T cells to the gray matter was further evaluated by inducing a small laser injury in layer 1 barrel cortex between day 16–20 post EAE induction. Laser injury recruited GFP + T cells into the cortical gray matter of the CXCR6 gfp/+ , but not, CXCR6 gfp/gfp mice over a period of 3–4 days. CXCL16, the ligand for CXCR6, was induced in the spinal cord as well as the brain of the active and recovering EAE mice. We conclude that in EAE, CXCR6 is not required for localization of T cells to spinal cord white matter, but is necessary for T cell entry into brain gray matter. Funding: NIH grants and Collaborative MS Research Center Award to M.D., J.L. , J. Salzer.