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Human light chain V gene repertoires and transcription
Author(s) -
Schoettler Nathan,
Kalinina Olga,
Ni Dongyao,
Weigert Martin
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.2_supplement.419
Subject(s) - immunoglobulin light chain , gene , microbiology and biotechnology , biology , microarray analysis techniques , microarray , population , messenger rna , gene expression , genetics , antibody , medicine , environmental health
We have developed a microarray to study receptor light chain V gene repertoires in human B cells. Repertoires of naïve B cells have been determined at the mRNA level using the microarray, and single cell RT‐PCR has been used to determine the expressed light chain V gene repertoire. We have shown that the microarray V gene probe intensities correspond to their frequency in the expressed B cell pool. But the microarray shows that certain transcribed light chain V genes are not translated into complete light chains; two V lambda genes that are highly overrepresented at the mRNA level in all individuals are underrepresented in light chain databases and in the population of B cells studied with the single cell method. While these V lambda genes can rearrange to form functional light chains, they do so rarely according to the single cell data. Instead, the mRNA transcripts from these genes come from an unrearranged V gene and are found in approximately 30% of naïve B cells. Interestingly, these V region transcripts have the potential to be translated to form a V protein that lacks J and C regions. The role of these V proteins in differentiation and receptor editing will be discussed. This research was supported by the Gwen Knapp Center for Lupus and Immunology Research and NIH Grant GM02964‐29